Western blotting is an affordable process that reliably answers important basic questions regarding proteins of interest. To watch for ways to improve and refine routine practices and procedures, as time savings in these daily repetitive activities can add up quickly. Using time in the lab efficiently promotes success in research with faster generation of key data leading to scientific breakthroughs. How much time do you spend finding the right exposure settings for Western blots? Find out how the right CCD imager can help you work more efficiently, freeing valuable resources to drive your research forward, faster. In this eBook, we explore these solutions in detail and the advantages they offer researchers conducting western blotting in the lab.Ĭytiva’s ImageQuant™ 800 imager reveals hidden detail in this image. The Amersham ImageQuantTM 800 GxP biomolecular imagers, developed by Cytiva, offer many benefits for chemiluminescence and fluorescence detection, including enhanced sensitivity, resolution and data security. The choice of imaging solution therefore depends on the protein detection method, as well as the advantages and limitations of each solution. While the advent of X-ray film and digital imaging solutions (e.g., charge-coupled device (CCD) camera- based imagers and scanner-based systems) has transformed western blot detection, successful imaging requires the combined use of an accurate protein detection technique and imaging device. Understanding 21 CFR Part 11 and its application in analytical labs 35Ī western blot is a common analytical technique used to detect specific proteins within a sample, evaluate the size of a particular protein and/or to determine the level of protein expression. Traceability in Western blot imaging: Considerations for GxP 31 Western blot: the secrets to success in a GxP lab 27 ImageQuant™ 800 for lateral flow assay development using dyes across spectrum 22 Optimizing chemiluminescence and fluorescence imaging 22
![western blot quantification imagej and ccd imager western blot quantification imagej and ccd imager](http://www.lukemiller.org/journal/journal_pics/Westerns/Img11_box1.gif)
ImageQuant™ 800 for lateral flow assay development using dyes across the spectrum 14įluorescence reveals hidden details in flowers that attract pollinators 19 Optimize CCD imager signal-to-noise, without the guesswork 7 Innovative solutions for accurate western blot imaging
![western blot quantification imagej and ccd imager western blot quantification imagej and ccd imager](https://www.mdpi.com/nutrients/nutrients-14-03413/article_deploy/html/images/nutrients-14-03413-g005.png)
Ensure adherence to GxP, FDA 21 CFR Part 11, and EU GMP Annex 11 regulations for imaging workflows, including special focus on traceability, authenticity, audit trails, and user access.Extend dynamic range and avoid saturation.Improve image resolution, quality, and confidence for immunoblots as well as other research applications, including cell analysis, host cell protein ELISAs, and DNA gels.Work more efficiently with the right imager, including optimizing exposure times, automating signal-to-noise optimization, and scheduling.In particular, it provides valuable advice to: This resource guide on biomolecular imagers explores current requirements of chemiluminescence and fluorescence detection, including enhanced sensitivity, resolution, and data security, and their solutions. While X-ray film and digital imaging solutions (e.g., charge-coupled device (CCD) camera-based imagers and scanner-based systems) transformed western blot detection, selecting the right imaging solution is critical to success and depends on the protein detection method and the advantages and limitations of each solution. Successful detection and imaging of Western blots requires the combined use of an accurate protein detection technique and imaging device.